To detect the precence of human papillomavirus (HPV) DNA in penis cancer, 28 specimens from infiltrating epidermoid carcinoma were studied by Dot-blot analysis. All samples were from Paraguayan patients ranging in age from 30 to 80 years. Of these 14 (50 percent), contain DNA sequence homology. Nine samples were positive for Hpv type 16. Five samples for HPV type 18. No HPV type 11, 33 and 52 were detected in our samples