Strains of Trypanosoma cruzi isolate in Paraguay from patients and vectors were examiqned by restriction endonuclease analysis of K-DNA using Eco RI, Hae III, Hinf I, Msp I. Four different types of schizodeme profiles were identified in 11 isolates. The firs group has much simpler profiles than ther groups. There were Homogeneous profiles in the 3 other gruops, however, the profiles could be distinguished from each other. Standard striansfrom Brasil, Chile and Colombia were included in the schizodeme comparison. The schizodeme profiles of group 1 from Paraguay can not be matched with any of the standard strains in this study. Those of group 3 and 4 shared more features with those of Brazilian standard strains (Brazilian Z2)