The plasmid pSP65 which has cloned the complete HBV sequence, was amplified by transformation of coli (DH5). The HBV sequence was then labeled for its utilization as a probe in the HBV DNA detection in serum of patients with HBsAg positive, with no evident physical symptoms of the disease. The probe was labeled using digoxigenin, an enzymatic method. Sixteen samples were tested. Each sample was deproteinated by extraction with phenol-chorofrom in order to obtain Dane particles and then tested by dot bot. All samples yielded negative. This results suggest that the patients positive for HBsAg and who do not present acute symptoms nor high GOT and GPT levels in sera, lack HBV sequences in bloodstream