Proteins from Toxoplasma gondii were separated by SDS-PAGE and then transferred to a nitrocellulose membrane. Antigenic components were then detected by human sera with previously known Toxoplasma antibody levels. Peroxidase-conjugated anti-human immunoglobulins were used for the separated identification...
Microbiologic study were done on a sample isolated from the end of a catheter used in a child who had devepoped acute bacterial meningitis after several admissons to hospital. The isolate was identified as Klebsiella pneumoniae which showed a remarkable multiresistance to the most common antibiotics used...
Trisomy of chromosome 8 shows a characteristic phenotype which makes clinical diagnosis possible. This abnormality has been attributed to a duplication of the segment 8q22. In this work, it is reported a caseof partial trisomy of chromosome 8 which showed several abnormalities that are similar to those d...
This is the first research effort investigating cardiovascular risk in Paraguay. We report results of our ongoing cross-sectional prevalence study of riskfactors for coronary heart disease in the first 497 of 2,000 adult volunteers recruited from a population-based sample of households selected by a rand...
In an attempt to clone some specific parasite antigen genes, a Trypanosoma cruzi cDNA library was constructed in lambda gt11. Efficient isolation of undegraded mRNA from total RNA was obtained which is a critical step for the construction of representative cDNA libraries. Lambda gtll recombinant phages w...
Amonoclonal antibody (MoAb), designated TCY-3, against Trypanosoma cruzi was obtained from spleen cells of BALB/c mice immunized with crude soluble antigens of T. curzi trypomastigotes. This MoAb Showed no cross-reactivity with 12 other heterologous parasite antigens, including promastigotes of Leishmani...
An indirect agglutination test using Trypanosoma cruzi antigen-coated gelatin particles was employed to Trypanosomiasis in Paraguay. Results with this test were quite comparable to those obtained with enzyme-linked immusorbent assay (Elisa). Furthermore, nonspecific reaction to the gelatin particles alon...
In Paraguay, there are no updated data about mean values of weight and height in adults. The Instituto de Investigacion en Ciencias de la Salud (IICS) and the German Techinical Cooperation (GTZ) performed a study on cardiovascular risk factors in Asunción and metropolitan area. The aim of that study was...
Velázquez, Graciela;
Pozzoli, Lourdes;
Guillén, Ivalena;
Meza, Teresa;
Ferro, Esteban;
Kaspar, Peter;
Canese, Andrés;
Gómez, Francisca;
Villagra, Verónica;
González, Lidia;
Alvarez, Mercedes;
Beltrán, Miguela;
Meyer, Teresa;
Plans, José;
Zoulek, Gert.
A quality conrol assesssment laboratories of clinical analysis was performed in March 1989. Glucose, urea, bilirubin, and GOT were determined in a sample of the same control serum. Statistical evaluation of the results showed that the coefficient of variation (CV) of each parameter was much higher than t...
