Blood trnasfusion is the second most importnatmechanism of transmission of Chagas'disease in both endemic and non-endemic areas. In Latin America, more than 20,000 cases of transmission of the disease, trough blood transfusion, may be occurring. Gentian Violet is currently the only available trypanicidal...
The plasmid pSP65 which has cloned the complete HBV sequence, was amplified by transformation of coli (DH5). The HBV sequence was then labeled for its utilization as a probe in the HBV DNA detection in serum of patients with HBsAg positive, with no evident physical symptoms of the disease. The probe was ...
Trypanosoma cruzi amastigotes derived from fibroblast cultures and from cell- free liquid mediun were comparatively evaluated as solid-phase of Elisa for Chagas' disease. Amastigotes were obtained from the high- and low-virulent clones of the Talahuen strain and fron the G-1 strain. They were sonicated a...
chromosomal DNA from a Paraguayan Trypanosoma cruzi isolate was extracted and sonicated. The fragments were cloned and some clones were size-selectd and sequenced. Two primer pairs TC22 and TC4 were synthesized and applied to other Paraguayan T. cruzi isolates and two Leishmania mexicana amazoniensis str...
In order to establish the course of infection, virulence and pathogenicity, a Paraguayan Trypanosoma cruzi strain isolated from a 12 year old patient in 1989, Balb/c mice were inoculated, and the number of parasites were determined by direct parasitemia (micromethod). Pathogenicity were tested by perform...
The polymerase chain reaction (PCR) was usedto evaluate the course of treatment of an acutely infected patient with Trypanosoma cruzi. The reactions were performed using a primer pair (TCZ1 and TCZ2) which prime the amplification of a highly repetive 195-bp T. cruzi genomic DNA. Treatment with benzonidaz...
Complex II (Succinate-ubiquinone oxidoreductase) is an importnat enzyme complex for the tricarboxylic acid cycle and the aerobic respiratory chain of motochondria and procaryotic organisms (12, 24). Complex II catalyzes the oxidation of succinate to fumarate (Succinate dehydrogenase: sdh) and transfers i...
Three Cabus apella monkeys were infected with 3.5 x 10 sobre 5 cells of Trypanosoma cruzi Y strian in order to evaluate the parasitemia and to study the behavior of the parasites in the mammalian host for a one-year period. The parasitemia was determined by hemoconcentraction. In the there monkeys, circu...
Feces from 64 Cebus apella monkeys were examined by the MGL method. The monkeys have been kept in captivity at IICS. Larvae resembling those of Strongyloides were found in 25 percent of the animals, eggs resembling thosse of Strongyloide in 39 percent of the animals and eggs resembling those of Cestode ...
From April 1987 to December 1990, a total of 3895 persons participated in our anonymous HIV-antybody prevalence study in groups at risk for sexually-acquired HIV infection. Male travestied prostitutes had the highest HIV-antibody seroprevalence (> 30 precent). Seventy five percent of all known male trave...
